Genetic Heterology between Escherichia coli k12 and a Smooth Strain of E. coli
نویسندگان
چکیده
منابع مشابه
Genetic analysis of the histidine operon in Escherichia coli K12.
I N Salmonella typhimurium a cluster of nine genes, behaving as a single operon (AMES and GARRY 1959) specify the enzymes which carry out the ten reactions involved in the biosynthesis of histidine; one of the nine enzymes is bifunctional (LOPER, GRABNAR, STAHL, HARTMAN and HARTMAN 1964). The histidine operon is contained in a linear segment of deoxyribonucleic acid (DNA) estimated by AMES, GOL...
متن کاملHomologous recombination involving a large heterology in Escherichia coli.
Recombination between two different deletion alleles of a gene (neo) for neomycin and kanamycin resistance was studied in an Escherichia coli sbcA- recB-C- strain. The two homologous regions were in an inverted orientation on the same plasmid molecule. Kanamycin-resistant plasmids were selected and analyzed. The rate of recombination to form kanamycin-resistant plasmids was decreased by mutatio...
متن کاملPurification, crystallization and properties of porphobilinogen deaminase from a recombinant strain of Escherichia coli K12.
Porphobilinogen deaminase has been purified and crystallized from an overproducing recombinant strain of Escherichia coli harbouring a hemC-containing plasmid which has permitted the purification of milligram quantities of the enzyme. Determination of the Mr of the enzyme by SDS/polyacrylamide-gel electrophoresis (35,000) and gel filtration (32,000) agrees with the gene-derived Mr of 33,857. Th...
متن کاملA physical map of the Escherichia coli K12 genome.
A physical map of a genome is the structure of its DNA. Construction of such a map is a first step in the complete characterization of that DNA. The restriction endonuclease Not I cuts the genome of Escherichia coli K12 into 22 DNA fragments ranging from 20 kilobases (20,000 base pairs) to 1000 kilobases. These can be separated by pulsed field gel electrophoresis. The order of the fragments in ...
متن کاملIsolation and characterization of cysK mutants of Escherichia coli K12.
cysK mutants, deficient in O-acetylserine sulphydrylase A [O-acetyl-L-serine acetate-lyase (adding hydrogen-sulphide); EC 4.2.99.8], were isolated as strains resistant to selenite or giving a black colour reaction on bismuth citrate indicator medium. All were resistant to the inhibitor I,2,4-triazole. Four independent mutants were found which possessed lowered levels of O-acetylserine sulphydry...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1969
ISSN: 0022-1287
DOI: 10.1099/00221287-57-1-115